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                Reagent Center

                Spin Column Method

                • Biospin Fungus Genomic DNA Extraction Kit MORE
                  Product Details:

                  Kit Components 

                  Cat#

                  BSC14S1

                  BSC14M1

                  Components

                  50Tests

                  100Tests

                  LE Buffer

                  20 ml

                  40 ml

                  DA Buffer

                  7.5 ml

                  15 ml

                  E Binding Buffer

                  14ml

                  (add 28 ml absolute ethanol before use)

                  28ml

                  (add 56 ml absolute ethanol before use)

                  G Binding Buffer

                  25ml

                  50ml

                  Wash Buffer

                  25.2ml

                  (add 37.8ml absolute ethanol before use)

                  50.4ml

                  (add 75.6ml absolute ethanol before use)

                  Elution Buffer

                  10ml

                  20ml

                  Spin column

                  50

                  100

                  Handbook

                  1 copy

                  1 copy

                  Introduction

                      The kit provides a very simple, fast and economic way for the isolation of pure high–molecular-weight genomic DNA from fungus, adopting Genomic DNA Buffer Set. The simple purification procedure, based on the remarkable selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA in less than 1 hour. It requires no expensive equipment, involves only few steps, and completely avoids the use of toxic and hazardous reagents such as phenol and chloroform. In general, 2.5-30 μg genomic DNA can be acquired from up to 100 mg tissue or up to 5ml yeast culture by using Biospin Fungus Genomic DNA Extraction Kit.

                      The pure DNA can be applied extensively in PCR/Real-time PCR, sequencing, Southern blot, mutant analysis, SNP and the others.

                  Product Features:

                  Storage

                  • The kit should be stored at 15-25℃.
                  • All reagents, when stored properly, are stable for 18 months.

                  Apparatus and Materials to Be Supplied by the User

                  *  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

                  *  Microcentrifuge capable of 14,000g              * Absolute ethanol     * Vortex mixer

                  Important notes

                  1. Please add absolute ethanol to Wash Buffer and mix thoroughly before the first use.
                  2. Please add absolute ethanol to E Binding Buffer and mix thoroughly before the first use.
                  3. LE Buffer may form precipitates upon storage. If a precipitate has formed, incubate the buffer at 37°C until the precipitate has fully dissolved.
                  4. Prepare Sorbitol buffer: 18.217 g Sorbitol and 3.7224 g EDTA-2Na, dissolved in 100 ml of pure water.
                  Experimental data:

                  Analysis DNA

                  • Absorbance analysis

                  Get some DNA,diluted in a advisable factor with elution buffer. Survey the OD260   ,OD280 and OD320.

                  Expressions:concentration(μg/ml)=50×OD260×dilution fact Target:                2.0≥OD260-320/ OD280-320≥1.7

                  Notice: 1.0≥OD260≥0.1, the result of ratio is much reliable.

                  • Agarose Gel Analysis 0.81Agarose gel

                  Example 1:

                  Manual download
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